Microscopy/ResolveRT

The Microscopy Option (or so called ResolveRT) is the comprehensive option for visualization of microscopy data. It includes specific readers for microscopy data as well as a powerful 3D image deconvolution suite.
The Microscopy Option comes with several examples and step-by-step tutorials to easily learn how to enhance images with filters and the deconvolution process. This option includes the Filament Editor for vessel and neuron tracing and quantitative tools for micro-vascular network studies.

Platforms
Windows 32-bit, Windows 64-bit, Mac OS, Linux

Features


Advanced Tools for Microscopymicroscopy1
The Microscopy Option is the module dedicated to microscopy data
visualization and analysis. It provides import/export modules for all major
3D-image and microscopy data exchange format, and a complete
image deconvolution module.

  • Microscopy file readers (see File Formats)
  • Blind and non-blind deconvolution
  • Robust maximum likelihood algorithms
  • Fast convergence through overrelaxation
  • Support for wide-field and confocal microscopes
  • Computational PSF estimation
  • PSF extraction from bead measurements
  • Correction of intensity attenuation (Z-drop)
  • Background correction and flat-field correction
  • Support for multi-processor systems
  • Job queue for batch processing

 

 


Extract Neuron and Vessel Networksmicroscopy2
With applications in fluid dynamics, biomechanics, molecular simulation
and biomedicine, Amira´s vector field visualization offers high performance
and quality for representing simulation output or experimental 3D data sets.

  • Automatic skeletonization
  • Interactive filament tracing
  • Filament network labeling and editing
  • Automatic branching hierarchy detection
  • Automatic loop detection
  • Thick slice and volume rendering for tracing purposes.
  • Dedicated editor with a clear workflow

 

 

 

 

File Formats

Extension Read/Write

Bio-Rad Confocal simple uncompressed format for 3D image stacks

pic

r

Leica 3D TIFF contains 3D image data with voxel sizes

tif

r

Leica Binary 3D image stacks, time series, and meta information

lei

r

Leica Image 3D image stacks, time series, and meta information

lif

r

Leica Slice Series contains list of 2D TIFF files and meta information

info

r

MRC MRC file format for electron microscopy

mrc/rec

rw

Metamorph STK special TIFF variant for 3D image stacks

tif

r

Olympus OIB/OIF binary file format (windows only)

oib/oif

r

Zeiss LSM 3D raster image format

lsm

r